The International CCN Society

Log in | Register

Contact Us

Secretary of the ICCNS Scientific Board

Satoshi Kubota

Satoshi Kubota, D.D.S., Ph.D.
Department of Biochemistry and Molecular Dentistry
Okayama University Graduate School
of Medicine, Dentistry and Pharmaceutical Sciences
2-5-1 Shikata-cho
Okayama 600-8525 JAPAN

Present Position

  • Professor and Chairman
    Department of Biochemistry and Molecular Dentistry
    Okayama University Graduate School of Medicine,
    Dentistry and Pharmaceutical Sciences
  • Adjunct Research Assistant Professor of Medicine
    Division of Infectious Diseases and Environmental Medicine Thomas Jefferson University


  • International CCN Society (liaison for Bone and Cartilage Societies)
  • Japanese Society of Cartilage Metabolism (Director)
  • Japanese Association for Oral Biology (Councilor)
  • Okayama Dental Society (Director)
  • The Japanese Biochemical Society (councilor)
  • The Molecular Biology Society of Japan (councilor)
  • Japanese Society for Bone and Mineral Metabolism (councilor)
  • Japanese Cancer Association

Editorial Boards

  • Journal of Cell Communication and Signaling, Molecular Signaling Section Editor
  • Journal of Orthopaedic Science
  • Journal of Bone and Mineral Metabolism


  • 1996 Junior Investigator Award, Thomas Jefferson University
  • 2006 Scientific Award, Okayama Dental Society
  • 2012 Research Promotion Award, International CCN Society

Research Themes

  • Transcriptional and post-transcriptional regulation of CCN2 and the other CCN family genes
  • Role and utility of CCN2 and other CCN family members in bone and cartilage regeneration
  • Molecular network around the CCN family members

Selected Recent Publications

  • Nishida T, Kubota S, Aoyama E, Yamanaka N, Lyons KM, Takigawa M.
    Low-intensity pulsed ultrasound (LIPUS) treatment of cultured chondrocytes stimulates production of CCN family protein 2 (CCN2), a protein involved in the regeneration of articular cartilage: mechanism underlying this stimulation.
    Osteoarthritis Cartilage 2016; in press.
  • Murase Y, Hattori T, Aoyama E, Nishida T, Maeda-Uematsu A, Kawaki H, Lyons KM, Sasaki A, Takigawa M, Kubota S.
    Role of CCN2 in amino acid metabolism of chondrocytes.
    J Cell Biochem. 2016;117:927-937.
  • Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Matsumoto T, Young MF, Olsen BR, Kuboki T.
    Fluocinolone acetonide is a potent synergistic factor of TGF-β3-associated chondrogenesis of bone marrow-derived mesenchymal stem cells for articular surface regeneration.
    J Bone Miner Res 2015;30:1585-96.
  • Abd El Kader T, Kubota S, Nishida T, Hattori T, Aoyama E, Janune D, Hara ES, Ono M, Tabata Y, Kuboki T, Takigawa M
    The regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarthritis models in vivo.
    Bone 2014; 59:180-188.
  • Maeda-Uematsu A, Kubota S, Kawaki H, Kawata K, Miyake Y, Hattori T, Nishida T, Moritani N, Lyons KM, Iida S, Takigawa M
    CCN2 as a novel molecule supporting energy metabolism of chondrocytes.
    J. Cell. Biochem. 2014; 115:854-865.
  • Nishida T, Kubota S, Aoyama E, Takigawa M
    Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus.
    Osteoarthritis Cartilage 2013; 21: 700-709.
  • Sumiyoshi K, Kubota S, Ohgawara T, Kawata K, Abd El Kader T, Nishida T, Ikeda N, Shimo T, Yamashiro T, Takigawa M
    Novel role of miR-181a in cartilage metabolism.
    J. Cell. Biochem. 2013; 114: 2094-2100.
  • Kawata K, Kubota S, Eguchi T, Aoyama E, Moritani N, Kondo S, Nishida T, Takigawa M
    Role of low-density lipoprotein receptor related protein 1 (LRP1) in CCN2/connective tissue growth factor (CTGF) protein transport in chondrocytes.
    J Cell Sci. 2012; 25: 2965-2972.
  • Aoyama E, Kubota S, Takigawa M
    CCN2/CTGF binds to fibroblast growth factor receptor 2 and modulates its signaling.
    FEBS Letters 2012; 586: 4270-4275.
  • Kodama T, Takehara T, Hikita H, Shimizu S, Shigekawa M, Tsunematsu H, Li W, Miyagi T, Hosui A, Tatsumi T, Ishida H, Kanto T, Hiramatsu N, Kubota S, Takigawa M, Tomimaru Y, Tomokuni A, Nagano H, Doki Y, Mori M. Hayashi N
    Increases in p53 expression induce CTGF synthesis by mouse and human hepatocytes and result in liver fibrosis in mice.
    J Clin Invest. 2011; 121: 3343-3356.
  • Nishida T, Kubota S, Aoyama E, Janune D, Maeda A. Takigawa M
    Effect of CCN2 on FGF2-Induced Proliferation and MMP9 and MMP13 Productions by Chondrocytes.
    Endocrinology 2011; 152: 4232-4241.
  • Kawaki H, Kubota S, Suzuki A, Suzuki M, Kohsaka K, Hoshi K, Fujii T, Lazar N, Ohgawara T, Maeda T, Perbal B, Takano-Yamamoto T, Takigawa M
    Differential roles of CCN family proteins during osteoblast differentiation: involvement of Smad and MAPK signaling pathways.
    Bone 2011. 49: 975-989.
  • Janune D, Kubota S, Nishida T, Kawaki H, Perbal B, Iida S, Takigawa M
    Novel effects of CCN3 that may direct the differentiation of chondrocytes. FEBS Lett. 2011; 585: 3033-3040.
  • Nishida T, Emura K, Kubota S, Lyons KM, and Takigawa M
    CCN family 2/connective tissue growth factor (CCN2/CTGF) promotes osteoclastogenesis via induction of and interaction with dendritic cell-specific transmembrane protein (DC-STAMP).
    J Bone Miner. Res. 2011; in press .
  • Mukudai Y, Kubota S, Eguchi T, Sumiyoshi K, Janune D, Kondo S, Shintani S, and Takigawa M
    A coding RNA segment that enhances the ribosomal recruitment of chicken ccn1 mRNA.
    J Cell. Biochem. 2010; 111: 1607-1618. .
  • Kawaki H, Kubota S, Aoyama E, Fujita N, Hanagata H, Miyauchi A, Nakai K, and Takigawa M
    Design and utility of CCN2 anchor peptide aptamers.
    Biochimie 2010; 92: 1010-1015 .
  • Takeuchi H, Kubota S, Murakashi E, Zhou Y, Endo K, Ng PS, Takigawa M, and Numabe Y
    Nicotine-induced CCN2: from smoking to periodontal fibrosis.
    J Dent. Res. 2010; 89: 34-39 .
  • Kawata K, Kubota S, Eguchi T, Moritani NH, Shimo T, Kondo S, Nishida T, Minagi S, and Takigawa M.
    Role of the low-density lipoprotein receptor-related protein-1 in regulation of chondrocyte differentiation.
    J Cell. Physiol. 2010; 222: 138-148 .
  • Ohgawara T, Kubota S, Kawaki H, Kondo S, Eguchi T, Kurio N, Aoyama E, Sasaki A, Takigawa M.
    Regulation of chondrocytic phenotype by micro RNA 18a: involvement of Ccn2/Ctgf as a major target gene.
    FEBS Lett. 2009; 583: 1006-1010 .
  • Aoyama E, Hattori T, Hoshijima M, Araki D, Nishida T, Kubota S, and Takigawa M.
    N-terminal domains of CCN family 2/connective tissue growth factor bind to aggrecan.
    Biochem J. 2009; 420: 413-420 .
  • Mukudai Y, Kubota S, Kawaki H, Kondo S, Eguchi T, Sumiyoshi K, Ohgawara T, Shimo T, and Takigawa M.
    Posttranscriptional regulation of chicken ccn2 gene expression by nucleophosmin/B23 during chondrocyte differentiation.
    Mol. Cell. Biol. 2008; 28: 6134-6147 .
  • Eguchi T, Kubota S, Kawata K, Mukudai Y, Uehara J, Ohgawara T, Ibaragi S, Sasaki S, Kuboki T, and Takigawa M
    Novel transcription factor-like function of human MMP3 regulating CTGF/CCN2 gene
    Mol Cell Biol. 2008 Apr;28(7):2391-413. Epub 2008 Jan 2.
  • Kikuchi T, Kubota, S, Asaumi K, Kawaki H, Nishida T, Kawata K, Mitani S, Tabata Y, Ozaki T, and Takigawa M
    Promotion of bone regeneration by CCN2 incorporated into gelatin hydrogel
    Tissue Eng. 2008; in press
  • Fujisawa T, Hattori T, Ono M, Uehara J, Kubota, S, Kuboki T, and Takigawa M
    CCN family 2 / Connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes.
    Osteoarthritis Cartilage 2008; in press
  • Motoki T, Sugiura Y, Matsumoto Y, Tsuji T, Kubota S, Takigawa M, and Gohda E
    Induction of hepatocyte growth factor expression by maleic acid in human fibroblasts through MAPK activation.
    J. Cell. Biochem. 2008; in press
  • Koitabashi N, Arai M, Niwano K, Watanabe A, Endoh M, Suguta M, Yokoyama T, Tada H, Toyama T, Adachi H, Naito S, Oshima S, Nishida T, Kubota S, Takigawa M, and Kurabayashi M
    Plasma connective tissue growth factor is a novel potential biomarker of cardiac dysfunction in patients with chronic heart failure.
    Eur. J. Heart Failure 2008; in press
  • Ono M, Kubota S, Fujisawa T, Sonoyama W, Kawaki H, Akiyama K, Oshima M, Nishida T, Yoshida Y, Suzuki K, Takigawa M, and Kuboki T
    Promotion of hydroxyapatite-associated, stem cell-based bone regeneration by CCN2.
    Cell Transplantation 2008; in press
  • Kubota S, and Takigawa M
    Role of CCN2/CTGF/Hcs24 in Bone Growth.
    Int. Rev. Cytol. 2007; 147: 1-41
  • Kubota S, and Takigawa M
    CCN2 Molecule Page
    UCSD/Nature. The Signaling Gateway. 2007; AfCS ID A003961:
  • Kubota S, and Takigawa M
    CTGF (connective tissue growth factor).
    Atlas Genet Cytogenet Oncol Haematol. 2007; CTGFID40192.
  • Eguchi T, Kubota S, Kawata K, Mukudai Y, Ohgawara T, Miyazono K, Nakao K, Kondo S, and Takigawa M
    Different transcriptional strategies of ccn2/ctgf gene induction between human chondrocytic and breast cancer cell lines.
    Biochimie, 2007; 89: 278-288
  • Yanagita T, Kubota S, Kawata K, Kawaki H, Kondo S, Takano-Yamamoto T, Tanaka S. and Takigawa M
    Expression and physiological role of CCN4/Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes
    FEBS J. 2007; 274:1655-1665.
  • Koitabashi N, Arai M, Kogure S, Niwano K, Watanabe A, Aoki Y, Maeno T, Nishida T, Kubota S, Takigawa M, and Kurabayashi M
    Increased connective tissue growth factor relative to brain natriuretic peptide as a determinant of myocardial fibrosis.
    Hypertension 2007; 49:1120-1127.
  • Kondo S, Kubota S, Moritani N, Yosimichi G, Nishida T, Matsushita H, Matsumoto S, Kitoh T, Nakao K, Sugahara T, and Takigawa M
    Hypoxic Regulation of Stability of Connective tissue growth factor/CCN2 mRNA by 3'-untranslated region interacting with a cellular protein in human chondrosarcoma cells.
    Oncogene 2006; 25:1099-1110.
  • Shimo T, Kubota S, Eguchi T, Ibaragi S, Isowa S, Yoshioka N, Sasaki A, and Takigawa M
    Pathogenic role of connective tissue growth factor (CCN2/CTGF) in osteolytic metastasis of breast cancer.
    J. Bone Miner. Res. 2006; 21:1045-1059.
  • Yosimichi G, Kubota S, Nishida T, Kondo S, Yanagita T, Nakao K, Takano-Yamamoto T, and Takigawa M
    Roles of PKC, PI3K and JNK in multiple transduction of CCN2/CTGF signals in chondrocytes.
    Bone 2006; 38:853-863.
  • Kondo S, Tanaka N, Kubota S, Mukudai Y, Yosimichi G, Sugahara T, and Takigawa M
    Novel angiogenic inhibitor DN-9693 that inhibits post-transcriptional induction of connective tissue growth factor (CTGF/CCN2) by vascular endothelial growth factor (VEGF) in human endothelial cells.
    Mol. Cancer Ther. 2006; 5:129-137.